| 红脂大小蠹后肠挥发性物质的鉴定、触角电位和室内趋向实验 |
| |
| 引用本文： | 闫争亮,方宇凌,孙江华,张钟宁.红脂大小蠹后肠挥发性物质的鉴定、触角电位和室内趋向实验[J].昆虫学报,2004,47(6):695-700. |
| |
| 作者姓名： | 闫争亮 方宇凌 孙江华 张钟宁 |
| |
| 作者单位： | 中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京,100080 |
| |
| 基金项目： | 中国科学院知识创新工程重大项目 (KSCXI_SW_13)，中国科学院知识创新工程项目 (KSCX3_IOZ_0 4) |
| |
| 摘 要： | 对外来松树害虫红脂大小蠹Dendroctonus valens LeConte的信息化学物质进行了研究。通过GC-MS测定，鉴定出红脂大小蠹后肠挥发性物质中除了含有松树挥发性物质α-蒎烯、β-蒎烯、3-蒈烯、月桂烯和柠檬烯外，还含有马鞭草烯醇和马鞭草烯酮；利用触角电位技术，对马鞭草烯醇、马鞭草烯酮以及在林间引诱效果最好的3-(+)-蒈烯进行了触角电生理测试；利用Y-型双向选择嗅觉仪对这些物质进行了室内趋向实验。实验结果表明: 1 μg的马鞭草烯醇、马鞭草烯酮和3-(+）-蒈烯引起雌雄触角的电位反应分别达416 μV/470 μV、597 μV/630 μV和926 μV/1 099 μV。浓度为1 μL/mL的马鞭草烯酮引起红脂大小蠹的正趋向反应，而在100 μL/mL浓度下引起红脂大小蠹的负趋向反应；在1 μL/mL的浓度下，3-(+ )-蒈烯引起了红脂大小蠹正趋向反应，而马鞭草烯醇则对红脂大小蠹具有驱避作用,说明这些物质在红脂大小蠹搜寻寄主和调节虫口密度方面起着重要作用。
|
| 关 键 词： | 红脂大小蠹 后肠挥发性物质 触角电位 趋向反应 |
| 文章编号： | 0454-6296(2004)06-0695-06 |
| 修稿时间： | 2004年3月29日 |
Identification and electroantennal olfactory and behavioral tests of hindgut-produced volatiles of the red turpentine beetle, Dendroctonus valens LeConte( Coleoptera:Scolytidae) |
| |
| YAN Zheng-Liang,FANG Yu-Ling,SUN Jiang-Hua,ZHANG Zhong-Ning.Identification and electroantennal olfactory and behavioral tests of hindgut-produced volatiles of the red turpentine beetle, Dendroctonus valens LeConte( Coleoptera:Scolytidae)[J].Acta Entomologica Sinica,2004,47(6):695-700. |
| |
| Authors: | YAN Zheng-Liang FANG Yu-Ling SUN Jiang-Hua ZHANG Zhong-Ning |
| |
| Affiliation: | YAN Zheng-Liang,FANG Yu-Ling,SUN Jiang-Hua,ZHANG Zhong-Ning~* |
| |
| Abstract: | An exotic invasive pine pest, red turpentine beetle, Dendroctonus valens LeConte (Scolytidae), was studied. GC-MS analysis confirmed the existence of verbenol and verbenone as well as the host volatile s (α-pinene, β-pinene, 3-carene, myrcene and limonene) in hindguts of the beetle. Verbenol, verbenone and 3-(+)-carene, the most effective attractant in field test, all elicited electroantennal responses in EAG (electroantennogram) test. Indoor olfactory tests with a Y-type tube were conducted. Verbenone was attractive to D. valens at 1 μL/mL, and its effect could parallel that of 3-carene; however, 100 μL/mL of verbenone was somehow repellent to th e beetle. Verbenol was repellent to D. valens in all dosages. |
| |
| Keywords: | Dendroctonus valens hindgut volatiles electroantennogram taxis response |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《昆虫学报》浏览原始摘要信息 |
|
点击此处可从《昆虫学报》下载全文 |
