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12a-羟基鱼藤酮对斜纹夜蛾生殖力的影响及其作用机理
引用本文:李有志,杨海君,徐汉虹,刘仲华,高必达,张瑞峰,周志成.12a-羟基鱼藤酮对斜纹夜蛾生殖力的影响及其作用机理[J].昆虫学报,2009,52(3):267-273.
作者姓名:李有志  杨海君  徐汉虹  刘仲华  高必达  张瑞峰  周志成
作者单位:1. 湖南农业大学生物安全科学技术学院,长沙,410128
2. 华南农业大学农药与化学生物学教育部重点实验室,广州,510642
3. 湖南农业大学茶学教育部重点实验室,长沙,410128
4. 湖南省烟草公司,长沙,410007
摘    要:为探讨12a-羟基鱼藤酮对斜纹夜蛾Prodenia litura (Fabricius)生殖力的影响及其作用机理, 本文测定了该虫取食12a-羟基鱼藤酮后的产卵量。在用高效液相色谱仪确定其卵巢组织中存在12a-羟基鱼藤酮后, 通过MTT法测定该化合物对斜纹夜蛾卵巢细胞(PL细胞)的细胞毒性,并利用流式细胞仪检测该化合物对PL细胞细胞周期、膜电位、胞内钙离子浓度、线粒体膜电位的影响。结果表明:斜纹夜蛾幼虫取食12a-羟基鱼藤酮后产卵量下降80%以上, 并从卵巢组织中检测出该化合物。12a-羟基鱼藤酮对PL细胞增殖具有抑制活性, 其IC50为6.6 mg/L。该化合物将PL细胞周期阻滞于S期, 导致PL细胞膜电位、线粒体膜电位和细胞内游离钙离子浓度均显著升高。该化合物导致斜纹夜蛾繁殖力降低。12a-羟基鱼藤酮将PL细胞增殖阻滞于细胞周期的S期。同时, 该化合物对卵巢细胞具有较弱的毒杀活性, 导致一些卵巢细胞死亡。由于上述原因, 卵巢细胞数量逐渐减少而导致卵巢管萎缩。卵巢管的萎缩使卵巢管内的卵母细胞不能发育成卵细胞, 故其生殖力下降。

关 键 词:斜纹夜蛾  12a-羟基鱼藤酮  繁殖力  细胞毒性  细胞周期  钙离子浓度  线粒体膜电位  

Effect of 12a-hydroxyrotenone on the fecundity of Prodenia litura (Fabricius)(Lepidoptera: Noctuidae) and its mechanisms
LI You-Zhi,YANG Hai-Jun,XU Han-Hong,LIU Zhong-Hua,GAO Bi-Da,ZHANG Rui-Feng,ZHOU Zhi-Cheng.Effect of 12a-hydroxyrotenone on the fecundity of Prodenia litura (Fabricius)(Lepidoptera: Noctuidae) and its mechanisms[J].Acta Entomologica Sinica,2009,52(3):267-273.
Authors:LI You-Zhi  YANG Hai-Jun  XU Han-Hong  LIU Zhong-Hua  GAO Bi-Da  ZHANG Rui-Feng  ZHOU Zhi-Cheng
Abstract:Three special substrates were used in enzyme activity analysis to evaluate the effects of pH on the digestive protease activity in the midgut of locust Catantops pinguis (Stål). The results showed that the pH value in the midgut of locust had no difference between the sexes, which was 6.92±0.043 in male and 7.03±0.054 in female. Three serine digestive proteases in midgut were identified by three special substrates. The optimal pH of the active alkaline trypsin-like enzyme (BAPNA as the substrate) was 8.5 in the female and 10.5 in the male; the optimal pH of weak alkaline trypsin-like enzyme (TAME as the substrate) was 9.0 in the female and 9.5 in the male; the optimal pH of chymotrypsin-like enzyme (BTEE as the substrate) was 8.5 in the female and 8.0 in the male. The statistical results showed that pH affected the serine protease activity obviously (P<0.01), and the activity of the same protease in the female was remarkably different from that in the male (P<0.01), meaning the activity of trypsin-like enzyme in the female was higher than that in the male at the optimal pH, while the activity of chymotrypsin-like enzyme in the male was higher than that in the female at the optimal pH. In the range of pH in midgut, the female has a higher digestive protease activity than the male, suggesting that the female has a stronger capacity to deal with the food intake to get more nutrients for reproduction (forming eggs). The digestive proteases remain active over a wide pH range, which may give this locust an adaptive advantage of feeding on a wide range of plants in the field.
Keywords:Catantops pinguis  midgut  protease  activity  pH
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