| Bt杀虫蛋白对不同品系棉铃虫和中红侧沟茧蜂生长发育的影响 |
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| 引用本文： | 刘小侠,张青文,蔡青年,李建成,董杰.Bt杀虫蛋白对不同品系棉铃虫和中红侧沟茧蜂生长发育的影响[J].昆虫学报,2004,47(4):461-466. |
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| 作者姓名： | 刘小侠 张青文 蔡青年 李建成 董杰 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100094
2. 河北省农林科学院植物保护研究所,河北保定,071000 |
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| 基金项目： | 国家“973”项目 (G2 0 0 0 0 162 0 9)，国家科技攻关计划项目 ( 2 0 0 1BA5 0 7A 11 0 2 ) |
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| 摘 要： | 以棉铃虫Helicoverpa armigera (Hübner)室内敏感品系和田间品系为寄主，研究了亚致死浓度的Bt杀虫蛋白对中红侧沟茧蜂Microplitis mediator (Haliday)生长发育的影响。结果表明： 当寄主一直取食，或者在被寄生前12小时开始取食含Bt杀虫蛋白浓度为0，0.5，1.0， 2.0，4.0，8.0 μg/g的饲料时，与对照相比，中红侧沟茧蜂的卵-幼虫历期延长，茧重和成虫体重降低，成虫寿命缩短,但对茧期没有明显影响。Bt杀虫蛋白能有效抑制两个棉铃虫品系幼虫的生长，显著降低棉铃虫蛹重；当Bt蛋白浓度为4.0 μg/g时，显著降低棉铃虫化蛹率。用转双基因抗虫棉SGK321（表达Cry1A+CpTI蛋白）饲喂两个棉铃虫品系初孵幼虫，室内品系的第2、3、4和5天校正死亡率分别为48.5%、87.8%、96.6%和 95.8%，显著高于田间品系（30.9%、59.6%、80.9%及86.1%）。本研究表明，不论是田间品系还是室内品系，棉铃虫取食含Bt杀虫蛋白的饲料后，对中红侧沟茧蜂的生长发育都具有显著的负面作用。
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| 关 键 词： | 棉铃虫 中红侧沟茧蜂 Bt杀虫蛋白 转基因抗虫棉 生长发育 |
| 文章编号： | 0454-6296(2004)04-0461-06 |
| 修稿时间： | 2003年12月18 |
Effect of Bt protein on development of different strains of the cotton bollworm, Helicoverpa armigera ( Hübner ) and the parasitoid, Microplitis mediator ( Haliday ) |
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| LIU Xiao_Xia.Effect of Bt protein on development of different strains of the cotton bollworm, Helicoverpa armigera ( Hübner ) and the parasitoid, Microplitis mediator ( Haliday )[J].Acta Entomologica Sinica,2004,47(4):461-466. |
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| Authors: | LIU Xiao_Xia |
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| Affiliation: | LIU Xiao_Xia~ |
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| Abstract: | Two strains of the cotton bollworm, Helicoverpa armigera (Hübner), one is a susceptible laboratory strain and the other is collected from Bt cotton fields, were used as the insect hosts to study the effect of Bt insecticidal protein on the development of the parasitoid, Microplitis mediator (Haliday). The results showed that the larval duration of the parasitoid was delayed, and the pupal weight, body weight of the newly emerged adult and adult longevity decreased significantly when the hosts larvae fed on diet containing Bt protoxin Cry1Ac at the concentrations of 0.5－8.0 μg/g in all time or from 12 h before parasitismtill pupation of the parasitoid . Compared with the control, the larval weight and pupal weight of H. armigera decreased significantly when the larvae fed on diet containing cry1Ac. Pupation rate of H. armigera decreased significantly in the treatment with diet containing Cry1Ac at 4 .0 μg/g. When H. armigera larvae fed on the transgenic cott on leaves expressing both Cry1A and CpTI proteins, the mortality of the laboratory strain was 48.5%－95.8% in 2－5 days after treatment, which was significantly higher than that of the field strain (30.9%－86.1%). It is concluded that feeding on diet containing Bt insecticidal protein, both strains of the cotton bollworm will have significantly negative effects on deve lopment of the parasitoid, M. mediator. |
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| Keywords: | Helicoverpa armigera Microplitis mediator Bt protoxin transgenic cotton development |
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