| 重金属在拟水狼蛛体内的分布及对其体内抗氧化酶活性的影响 |
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| 引用本文： | 张征田,庞振凌,梁子安,彭宇,杜瑞卿.重金属在拟水狼蛛体内的分布及对其体内抗氧化酶活性的影响[J].昆虫学报,2010,53(6):618-625. |
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| 作者姓名： | 张征田 庞振凌 梁子安 彭宇 杜瑞卿 |
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| 作者单位： | 1. 南阳师范学院生命科学与技术学院,河南南阳,473061
2. 湖北大学生命科学学院,武汉,430062 |
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| 基金项目： | 南阳师范学院青年项目，河南省重点学科项目 |
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| 摘 要： | 为了探明重金属在拟水狼蛛Pirata subpiraticus体内的分布及对其体内抗氧化酶活性的影响，本研究于2009年6月在河南南阳地区5种不同生境下，共采集50份土壤样本和300头拟水狼蛛样本，采用原子吸收光谱法测定了5种不同生境下雄雌拟水狼蛛体内重金属的分布、GSH含量以及GST，CAT和SOD的活性。结果表明：5个采集样点（S1， S2， S3， S4和S5） 拟水狼蛛体内重金属（Cd，Pb，Cu和Zn）的含量差异显著（P<0.05）。同一地点拟水狼蛛体内重金属（Cd，Pb，Cu和Zn）的含量不同身体部位差异显著（P<0.05），头胸部>足部>腹部，同一地点雄性拟水狼蛛重金属含量显著高于雌性（P<0.05）。在重金属胁迫下，重金属含量高（S1，S2，S3和S4）的拟水狼蛛体内GSH 含量显著高于参照组（S5），雄性GSH 含量高于雌性（P<0.05）。对于不同身体部位，GSH 含量差异显著，头胸部GSH 含量最高，其次为足部，腹部含量最低；GSH 含量与重金属含量显著正相关（r2=0.9854，P<0.05）。对于GST，CAT和SOD，重金属含量高则酶活性低，雄性酶活性显著低于雌性，不同身体部位酶活差异不显著；GST，CAT和SOD酶活性与重金属（Cd和Pb或者Pb）含量显著负相关。因此检测拟水狼蛛不同身体部位的酶活性变化就可知环境中重金属的污染程度，拟水狼蛛可以作为重金属污染的重要监测指示生物。
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| 关 键 词： | 拟水狼蛛 重金属污染 重金属含量 抗氧化酶 酶活性 |
Distribution of heavy metals in Pirata subpiraticus (Araneae: Lycosidae) and their effect on activities of antioxidant enzymes |
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| ZHANG Zheng-Tian,PANG Zhen-Ling,LIANG Zi-An,PENG Yu,DU Rui-Qing.Distribution of heavy metals in Pirata subpiraticus (Araneae: Lycosidae) and their effect on activities of antioxidant enzymes[J].Acta Entomologica Sinica,2010,53(6):618-625. |
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| Authors: | ZHANG Zheng-Tian PANG Zhen-Ling LIANG Zi-An PENG Yu DU Rui-Qing |
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| Abstract: | In order to study the distribution of heavy metals in wolf spider Pirata subpiraticus and their effect on activities of antioxidant enzymes, 50 soil samples and 300 individuals of P.. subpiraticus were collected from five different habitats in Nanyang City, Henan Province in June 2009. The contents of heavy metals (Cd, Pb, Cu and Zn), GSH level, and GST, CAT and SOD activities in different body parts of P. subpiraticus were measured using atomic absorption spectrometry. The results showed that the contents of Cd, Cu and Zn in P. subpiraticus were significantly different between four heavy metal polluted habitats (TBJK, TBTK, NYYT, and NYJG) and the control habitat (BTM, a nature reserve with less heavy metal pollution)(P<0.05), and also significantly different among different body parts in the same site (P<0.05), whereas the rank order was cephalothorax>legs>abdomen. Contents of Cd, Cu and Zn in the male were significantly higher than those in the female (P<0.05). The higher the content of heavy metals, the higher the GSH level. Moreover, the GSH level in the male was significantly higher than that in the female (P<0.05). In different body parts, the highest GSH level was found in the cephalothorax, followed by legs and abdomen. GSH level and heavy metal content correlated positively (r2=0.854, P<0.05), while significant negative correlations existed between heavy metal content (Cd and Pb or Pb) and activities of antioxidant enzymes. The enzyme activities in the male were significantly lower than those in the female. In the same site activities of antioxidant enzymes were not significantly different among different body parts. Therefore, the degree of heavy metal pollution can be known through testing the activity change of antioxidant enzymes of P. subpiraticus, and this wolf spider can be used as a very important bio-indicator for heavy metals pollution. |
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| Keywords: | Pirata subpiraticus heavy metal pollution heavy metal content antioxidant enzyme enzyme activity |
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