| 盐酸黄连素对蚜虫生物活性的研究 |
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| 引用本文： | 李明,曾唏,季祥彪,熊继文,康冀川.盐酸黄连素对蚜虫生物活性的研究[J].昆虫学报,1999,42(2):140-144. |
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| 作者姓名： | 李明 曾唏 季祥彪 熊继文 康冀川 |
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| 作者单位： | 贵州大学昆虫研究所,贵阳,550025 |
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| 基金项目： | 贵州省“八五”科技攻关项目 |
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| 摘 要： | 黄连素为黄连Coptis chinensis Franch体内的主要生物碱成份。用采自贵阳花溪附近的新鲜黄连主根提取盐酸黄连素,将粗品重结晶一次后,用 HITACHI-220紫外可见分光光度计测得一次重结晶产品含盐酸黄连素91%，黄连主根中的黄连素含量为11.56%。室内生物测定结果表明,盐酸黄连素对甘蓝蚜Brevicoryne brassicae (L.)具有很强的触杀作用和较好的拒食活性。1%盐酸黄连素的乙醇溶液点滴蚜虫，96 h死亡率高达92.5%,校正死亡率为91.4%, LC50值为(2.289±0.0125)g/L，回归方程Y=7.712+1.027X,相关系数r为0.958，达显著水平；1%盐酸黄连素的乙醇溶液处理叶片,24 h处理和对照叶面的蚜虫栖息率分别为20.6%和79.4%,拒食效果为74.04%, AFC50值为(4.055±0.0215) g/L ,回归方程Y＝9.115+1.719X，相关系数r为0.998，达极显著水平。
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| 关 键 词： | 黄连 盐酸黄连素 甘蓝蚜 触杀毒力 拒食活性 |
BIOACTIVITY OF BERBERINE HYDROCHLORIDE TO BREVICORYNE BRASSICAE(L.) |
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| Li Ming,Zeng Xi,Ji Xiangbiao,Xiong Jiwen,Kang Jichuan.BIOACTIVITY OF BERBERINE HYDROCHLORIDE TO BREVICORYNE BRASSICAE(L.)[J].Acta Entomologica Sinica,1999,42(2):140-144. |
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| Authors: | Li Ming Zeng Xi Ji Xiangbiao Xiong Jiwen Kang Jichuan |
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| Abstract: | Berberine is one of the alkloids in Coptis chinensis Franch. Berberine hydrochloride was extracted from fresh roots of Coptis chinensis from Huaxi, Guiyang. The extract was crystalized and the content was measured with spectrophotometer. The crystalized product contains 91% berberine hydrochloride. The percentage of berberine hydrochloride in the Coptis chinensis is 11.56%. The result of bioassay in laboratory showed that berberine hyddrochloride has very strong contact toxicity and relatively good antifeeding activity to Brevicoryne brassicae (Linnaeus).1% berberine hydrochloride solution of ethanol was prepared and used to treat the aphid typically. After 96 hours, mortality rate was 92.5%。Its rectified mortality and LC50 were 91.4% and (2.289±0.0215)g/L respectively. The regression equation is Y=7.712+1.027X. The coefficient of correlation is 0.958, reaching to the significance level. 1% berberine hydrochloride solution of ethanol was also used to treat cabbage leaves. Aphids were subsequently introduced on them. After 24 hours, the landing rate of aphids on the treated and control leave surfaces were 20.6% and 79.4% respectively. The antifeeding effect was 74.04%, AFC50＝(4.055±00215)g/L. The regression equation is Y＝9.115+1.719X. Coefficient of correlation is 0.998, at very significance level. |
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| Keywords: | Coptis chinensis berberine hgdrochloride Brevicoryne brassicae contact toxicity antifeeding activity |
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