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| 重金属Cd2+对拟水狼蛛体内金属硫蛋白含量及其生长发育的影响 | |
| 引用本文： | 张征田,余明玉,庞振凌,夏敏,杜瑞卿,杨方方,彭宇.重金属Cd2+对拟水狼蛛体内金属硫蛋白含量及其生长发育的影响[J].昆虫学报,2011,54(3):293-298. |
| 作者姓名： | 张征田 余明玉 庞振凌 夏敏 杜瑞卿 杨方方 彭宇 |
| 作者单位： | 1. 南阳师范学院生命科学与技术学院,河南南阳,473061 2. 南阳师范学院成人教育学院,河南南阳,473061 3. 湖北大学生命科学学院,武汉,430062 |
| 基金项目： | 南阳师范学院青年项目，河南省重点学科项目 |
| 摘 要： | 为了明确重金属Cd2+对拟水狼蛛Pirata subpiraticus体内金属硫蛋白(metallothionein, MT)含量及其生长发育的影响， 在室内条件下用5个不同浓度（0， 10， 20， 40和80 μg/g）的Cd2+培养的黑腹果蝇Drosophila melanogaster饲喂从5种不同生境下（S1， S2， S3， S4和S5）采集的拟水狼蛛性成熟雌蛛产卵孵化的幼蛛，待幼蛛性成熟后取所得雌蛛成蛛采用原子吸收光谱法测定了Cd2+ 诱导下拟水狼蛛体内金属硫蛋白含量及其存活率和生长率。结果表明： 食物中过量的Cd2+能够通过食物链进行传递并在拟水狼蛛体内积累， 积累量随黑腹果蝇培养基中Cd2+浓度的增加而增加，存在显著的浓度-效应关系。不同浓度的Cd2+能够诱导拟水狼蛛体内MT不同的表达，表达量与Cd2+浓度显著正相关(P<0.05)。当浓度低于20 μg/g时，污染点（S1， S2， S3和S4）拟水狼蛛体内MT表达量显著高于参照组S5（P<0.05）； 当高于20 μg/g时， 所有样点拟水狼蛛体内MT表达量差异不显著（P＞0.05）。拟水狼蛛存活率和成长率随着Cd2+浓度的升高呈下降趋势。据此认为，金属硫蛋白可能是蜘蛛耐受重金属污染的重要机制，与重金属具有一定浓度-效应关系。 |
| 关 键 词： | 拟水狼蛛 重金属污染 镉 金属硫蛋白 存活率 生长发育 |
Effects of cadmium on metallothionein content in Pirata subpiraticus(Araneae: Lycosidae)and its growth and development |
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| ZHANG Zheng-Tian,YU Ming-Yu,PANG Zhen-Ling,XIA Min,Du Rui-Qing,YANG Fang-Fang,PENG Yu.Effects of cadmium on metallothionein content in Pirata subpiraticus(Araneae: Lycosidae)and its growth and development[J].Acta Entomologica Sinica,2011,54(3):293-298. | |
| Authors: | ZHANG Zheng-Tian YU Ming-Yu PANG Zhen-Ling XIA Min Du Rui-Qing YANG Fang-Fang PENG Yu |
| Abstract: | In order to explore the effects of cadmium on metallothionein (MT) content of Pirata subpiraticus and its growth and development, offsprings of P. subpiraticus female adults collected from five different habitats were fed on Drosophila melanogaster reared under 5 different Cd2+ concentrations in the laboratory. After sexually matured, then the MT content in body, growth rate and survival rate of these female spiders were tested. The results showed that excessive cadmium in food can be transferred by food chain and accumulated in P. subpiraticus and the accumulation level increased with cadmium concentration increasing. The induced expression level of MT was positively related with Cd2+ concentration (P<0.05). When Cd2+ concentration was less than 20 μg/g, the expression level of MT in P. subpiraticus from polluted sites （S1, S2, S3 and S4） was significantly higher than that from the control site S5 (P<0.05). On the contrary， when Cd2+ concentration was higher than 20 μg/g, however, the expression levels of MT in P. subpiraticus from all sites had no significant difference. The survival and growth rates of P. subpiraticus decreased with increasing Cd2+ concentration. It is so concluded that MT plays an important role in tolerance mechanism in P. subpiraticus, and MT increases significantly in a concentration-dependent manner with heavy metals. |
| Keywords: | Pirata subpiraticus" target="_blank">Pirata subpiraticus')" href="#">Pirata subpiraticus heavy metal pollution cadmium metallothionein survival rate growth and development |
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