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| 亚洲玉米螟幼虫酚氧化酶原基因序列的生物信息学分析 | |
| 引用本文： | 冯从经,陆剑锋,黄建华,郭晓丽,桑守亮.亚洲玉米螟幼虫酚氧化酶原基因序列的生物信息学分析[J].昆虫学报,2009,52(10):1068-1077. |
| 作者姓名： | 冯从经 陆剑锋 黄建华 郭晓丽 桑守亮 |
| 作者单位： | 1. 扬州大学园艺与植物保护学院植物保护系,江苏扬州,225009 2. 合肥工业大学生物与食品工程学院,合肥,230009 3. 中国科学院上海生命科学研究院植物生理生态研究所,上海,200032 |
| 基金项目： | 国家自然科学基金，扬州大学科技创新培育基金 |
| 摘 要： | 酚氧化酶原PPO是昆虫免疫的关键酶, 本文从生物信息学角度对亚洲玉米螟Ostrinia furnacalis Guenée幼虫PPO进行分析, 为进一步研究其高级结构与功能的关系提供理论依据。利用我们已提交到GenBank的数据, 采用在线分析及MEGA4和RasMol软件对亚洲玉米螟酚氧化酶原（Of-PPO）的核苷酸和氨基酸序列、系统发生关系和蛋白质三级结构进行分析。结果表明: Of-PPO全长cDNA序列有2 686 bp, 包含一个2 079 bp的开放阅读框, 其推导的693个氨基酸序列中包含6个组氨酸残基构成的2个铜离子结合位点, 以及保守的硫羟酸酯区域。Of-PPO属于PPO2类群, 其N端不含信号肽, 无跨膜结构域区域, 无糖基化位点, 44个磷酸化位点均匀分布于整个多肽链中, 有2段序列可能形成卷曲螺旋, 有5个区域的氨基酸具较强疏水性, 其二级结构中α-螺旋占22.54%, 随机卷曲占56.79%。同源建模显示其三级结构为“α/β型”中的“滚筒结构”, 存在一个明显的空位, 可能与该酶催化活性有关。本文可为Of-PPO的实验研究和应用开发提供有价值的信息。 |
| 关 键 词： | 亚洲玉米螟 酚氧化酶原 生物信息学分析 三级结构 同源建模 |
Bioinformatical analysis of prophenoloxidase from larvae of the Asian corn borer,Ostrinia furnacalis Guenée(Lepidoptera:Pyralidae) |
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| FENG Cong-Jing,LU Jian-Feng,HUANG Jian-Hua,GUO Xiao-Li,SANG Shou-Liang.Bioinformatical analysis of prophenoloxidase from larvae of the Asian corn borer,Ostrinia furnacalis Guenée(Lepidoptera:Pyralidae)[J].Acta Entomologica Sinica,2009,52(10):1068-1077. | |
| Authors: | FENG Cong-Jing LU Jian-Feng HUANG Jian-Hua GUO Xiao-Li SANG Shou-Liang |
| Abstract: | Prophenoloxidase (PPO) is a key enzyme of insect immunity. In this study, the nucleotide sequence and deduced amino acid sequence of PPO cloned from the larvae of Ostrinia furnacalis were analyzed and predicted by the tools of bioinformatics in the following aspects: the composition of nucleotide sequences and amino acid sequences, signal peptide, trans-membrane topological structure, hydrophobicity or hydrophilicity, secondary structure, tertiary structure, etc. The results showed that the full-length cDNA of 0. furnacalis PPO (Of-PPO) consisted of 2 686 bp, containing an open reading frame (ORF) of 2 079 bp. Based on the 693 deduced amino acid sequences of Of-PPO, a putative thiolester site and two distinct copper binding regions were found, which included six histidine residues. Of-PPO belongs to the PPO2 group, and there is no signal peptide in the N-terminal region of the polypeptide chain. No possible transmembrane protein model was found. There was no O-glycosylation site in Of-PPO predicted with DictyoGlyc. Forty-four phosphorylation sites were predicted in the whole peptide by NetPhos 2.0 Server. Two regions of Of-PPO could form coil structures, and five regions were found with significant hydrophobic characteristics. The proportions of α-helix and random coil in the secondary structure of Of-PPO were 22.54% and 56.79% , respectively. The comparative modeling method was used to calculate the tertiary structure of Of-PPO, and the result showed that it was the "roller" shape, which belongs to the α/β type. The catalytic activity may relate with a large vacant space in Of-PPO structure analyzed by the RasMol software. This study may provide valuable information for further research on Of-PPO. |
| Keywords: | Ostrinia furnacalis prophenoloxidase bioinformatical analysis tertiary structure comparative modeling |
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