Phenolic Composition,Anti‐Inflammatory,Antioxidant, and Antimicrobial Activities of Alchemilla mollis (Buser) Rothm. |
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| Authors: | Gökçe Şeker Karatoprak Selen İlgün Müberra Koşar |
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| Affiliation: | 1. Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey;2. Department of Pharmaceutical Botany, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey;3. Department of Pharmacognosy, Faculty of Pharmacy, Eastern Mediterranean University, Gazima?usa, Mersin, Turkey |
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| Abstract: | The current study was designed to evaluate the antioxidant, anti‐inflammatory and antimicrobial activities of Alchemilla mollis (Buser ) Rothm . (Rosaceae) aerial parts extracts. Chemical composition was analyzed by spectrophotometric and chromatographic (HPLC) techniques. The antioxidant properties assessed included DPPH· and ABTS·+ radical scavenging, β‐carotene‐linoleic acid co‐oxidation assay. Antimicrobial activity was evaluated with disc diffusion and micro dilution method. In order to evaluate toxicity of the extracts, with the sulforhodamine B colorimetric assay L929 cell line (mouse fibroblast) was used. The anti‐inflammatory activities of the potent antioxidant extracts (methanol, 70% methanol, and water extracts) were determined by measuring the inhibitory effects on NO production and pro‐inflammatory cytokine TNF‐α levels in lipopolysaccharide stimulated RAW 264.7 cells. 70% methanol and water extracts which were found to be rich in phenolic compounds (184.79 and 172.60 mg GAE/g extract) showed higher antioxidant activity. Luteolin‐7‐O‐glucoside was the main compound in the extracts. Ethyl acetate and 70% methanol extracts showed higher antibacterial activity against Staphylococcus aureus and Salmonella enteritidis with MIC value of 125 μg/ml. 70% methanol extract potentially inhibited the NO and TNF‐α production (18.43 μm and 1556.22 pg/ml, respectively, 6 h). |
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| Keywords: |
Alchemilla mollis
Rosaceae
HPLC
Anti‐inflammatory activity Antioxidant activity Antimicrobial activity |
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