In vitro screening of Fe2+‐chelating effect by a Fenton's reaction–luminol chemiluminescence system |
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| Authors: | Mitsuhiro Wada Hiroaki Komatsu Rie Ikeda Talal A Aburjai Suleiman M Alkhalil Naotaka Kuroda Kenichiro Nakashima |
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| Affiliation: | 1. Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Faculty of Pharmacy, University of Jordan, Amman, Jordan;3. Faculty of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan |
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| Abstract: | In vitro screening of a Fe2+‐chelating effect using a Fenton's reaction–luminol chemiluminescence (CL) system is described. The luminescence between the reactive oxygen species generated by the Fenton's reaction and luminol was decreased on capturing Fe2+ using a chelator. The proposed method can prevent the consumption of expensive seed compounds (drug discovery candidates) owing to the high sensitivity of CL detection. Therefore, the assay could be performed using small volumes of sample solution (150 μL) at micromolar concentrations. After optimization of the screening conditions, the efficacies of conventional chelators such as ethylenediaminetetraacetic acid (EDTA), diethylentriaminepentaacetic acid (DETAPAC), deferoxamine, deferiprone and 1,10‐phenanthroline were examined. EC50 values for these compounds (except 1,10‐phenanthroline) were in the range 3.20 ± 0.87 to 9.57 ± 0.64 μM (n = 3). Rapid measurement of the Fe2+‐chelating effect with an assay run time of a few minutes could be achieved using the proposed method. In addition, the specificity of the method was discussed. Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | Fe2+ chelating effect Fenton's reaction luminol in vitro screening |
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