Subculturing of a polychlorinated biphenyl-dechlorinating anaerobic enrichment on solid media. |
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| Authors: | H D May A W Boyle W A Price nd and C K Blake |
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| Affiliation: | H D May, A W Boyle, W A Price, 2nd, and C K Blake |
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| Abstract: | An anaerobic culture capable of dechlorinating polychlorinated biphenyls was subcultured under strict anaerobic conditions on solid media containing sterilized river sediment. The dechlorination activity was transferred as a bacterial colony on a solid medium three times. After two transfers on solid medium, the culture was no longer methanogenic but still dechlorinated a mixture of tri- and tetrachlorobiphenyls. This demonstrates that anaerobic bacteria are responsible for the polychlorinated biphenyl dechlorination and can be grown without polychlorinated biphenyl on solid media. |
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