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The Molecular Weight and Other Biophysical Properties of Bromegrass Mosaic Virus
Authors:Larry E Bockstahler and  Paul Kaesberg
Abstract:Data are presented which show that bromegrass mosaic virus has a particularly low molecular weight and nucleic acid content. A molecular weight of 4.6 × 106 was calculated from the sedimentation coefficient, S°20,w = 86.2S, the diffusion coefficient, D20,w = 1.55 × 10-7 cm2/sec., and an assumed partial specific volume, UNK] = 0.708 ml/gm. The virus has a ribonucleic acid content of 1.0 × 106 atomic mass units. Electrophoresis experiments showed that the virus is stable in 0.10 ionic strength buffers in the pH range 3-6. Breakdown of the virus was observed outside this pH range. Some characteristics of the breakdown products are described.
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