Solute Accumulation in Tobacco Cells Adapted to NaCl |
| |
| Authors: | Binzel M L Hasegawa P M Rhodes D Handa S Handa A K Bressan R A |
| |
| Affiliation: | Department of Horticulture, Purdue University, West Lafayette, Indiana 47907. |
| |
| Abstract: | Cells of Nicotiana tabacum L. var Wisconsin 38 adapted to NaCl (up to 428 millimolar) which have undergone extensive osmotic adjustment accumulated Na+ and Cl− as principal solutes for this adjustment. Although the intracellular concentrations of Na+ and Cl− correlated well with the level of adaptation, these ions apparently did not contribute to the osmotic adjustment which occurred during a culture growth cycle, because the concentrations of Na+ and Cl− did not increase during the period of most active osmotic adjustment. The average intracellular concentrations of soluble sugars and total free amino acids increased as a function of the level of adaptation; however, the levels of these solutes did not approach those observed for Na+ and Cl−. The concentration of proline was positively correlated with cell osmotic potential, accumulating to an average concentration of 129 millimolar in cells adapted to 428 millimolar NaCl and representing about 80% of the total free amino acid pool as compared to an average of 0.29 millimolar and about 4% of the pool in unadapted cells. These results indicate that although Na+ and Cl− are principal components of osmotic adjustment, organic solutes also may make significant contributions. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
