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Genetic Diversity Analysis of Mycogone perniciosa Causing Wet Bubble Disease of Agaricus bisporus in China Using SRAP
Authors:Chunyuan Zhou  Dan Li  Liangchen Chen  Yu Li
Affiliation:Institute of Mycology/Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin, China
Abstract:In this study, 38 Mycogone perniciosa isolates of Agaricus bisporus from the main production areas in China were analysed for investigating the genetic diversity using sequence‐related amplification polymorphism (SRAP). A total of 132 polymorphic bands were obtained, ranging in size from 100 to 1700 base pairs. According to the dendrogram produced by the unweighted pair‐group method with arithmetic average of similarity coefficients from SRAP data, all the tested strains were divided into four clusters at a 71.6% similarity level. Strains 1–3 and 13–17 from, respectively, Xichong County of Sichuan Province and Yongchang County of Gansu Province were clustered in the same clade; strains 4–8 and 9–12 from, respectively, Long Hai City of Fujian Province and Luoyang City of Henan Province clustered in sa second clade; strains 18–21 from Wuhan City of Hubei Province grouped together in a third cluster distinct from the other strains.
Keywords:genetic diversity     Mycogone perniciosa     sequence‐related amplification polymorphism
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