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First Report of Peronospora belbahrii on Sweet Basil in Baja California Sur,México
Authors:Mirella Romero Bastidas  Liliana Saucedo Picazo  Bernardo Murillo Amador  Alejandra Nieto Garibay  Hever Latisnere Barragan  Luis G Hernandez Montiel
Affiliation:1. Centro de Investigaciones Biológicas del Noroeste, Calle Instituto Politécnico Nacional 195, La Paz, Baja California Sur, México;2. Facultad de Ciencias Agrícolas, Universidad Veracruzana, Xalapa, Veracruz, México
Abstract:In Baja California Sur, Mexico, a foliar disease occurred on sweet basil which seriously affected its quality and yield. The most common symptoms were yellowing and necrosis on leaves, caused by a downy mycelium growth on the lower leaf surface. Symptomatic leaves from two sampling sites were collected for morphological studies and molecular analysis of pathogen DNA. Based on morphological characteristics (sporangiophore size of 240–530 × 7–11 μm, branches of 5–8 order and a sporangia size of 27–31 × 21–25 μm) and molecular analysis (the GenBank blast of the PCR assays showed unique rDNA sequence data with 99% similarity to P. belbahrii), the pathogen was identified as Peronospora belbahrii, the causal agent of basil downy mildew. This is the first report of P. belbahrii affecting sweet basil in Mexico.
Keywords:Basil downy mildew     Ocimum basilicum        Peronospora belbahrii   
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