Isolation and Identification of Bacillus amyloliquefaciens IBFCBF‐1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper |
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| Authors: | Mengjun Zhang Jilie Li Airong Shen Shiyong Tan Zhun Yan Yongting Yu Zhaodong Xue Taimeng Tan Liangbin Zeng |
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| Affiliation: | 1. Key Laboratory of Cultivation and Protection for Non‐Wood Forest Trees, Central South University of Forestry and Technology, Changsha 410004, China;2. Hunan Academy of forestry, Changsha 410004, China;3. Key Laboratory of Plant Nutrition and Biological Fertilizer, Ministry of Agriculture, Changsha 410205, China;4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China |
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| Abstract: | In this study, 76 bacterial strains were isolated from the rhizosphere soil of pepper. Of these, 23 bacterial isolates capable of inhibiting Phytophthora capsici growth were selected. Among the antagonistic bacteria, one strain, IBFCBF‐1 showed the strongest antagonistic activity, and was identified as Bacillus amyloliquefaciens based on the results of 16S rRNA gene sequence analysis, physiological and biochemical testing, and morphological characteristics. When tested with a dual‐culture method and with laboratory greenhouse studies, the strain IBFCBF‐1 was found to be a potential biocontrol agent for controlling the plant pathogen, P. capsici. Moreover, it showed high efficiency and broad‐spectrum antifungal properties in vitro. Under greenhouse conditions, IBFCBF‐1 could significantly promote the growth of pepper seedlings, and was able to solubilize phosphate, and produce indole acetic acid (IAA) and ammonia. This study clearly demonstrated that IBFCBF‐1 is a potential candidate exhibiting phytophthora blight‐suppressive and plant growth‐promoting effects on pepper. |
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| Keywords: | antagonistic bacteria
Bacillus amyloliquefaciens
biocontrol agent
Phytophthora capsici
plant growth promoting |
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