A Thioredoxin-Mediated Activation of Glutamine Synthetase and Glutamate Synthase in Synchronous Chlorella sorokiniana |
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| Authors: | Tischner R Schmidt A |
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| Affiliation: | Pflanzenphysiogisches Institut, University of Göttingen, Göttingen, Federal Republic of Germany;Botanisches Institut, University of Munich, Munich, Federal Republic of Germany |
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| Abstract: | The effects of thioredoxin, dithioerythrol, and mixtures of both on enzymes involved in N metabolism of Chlorella sorokiniana have been studied. Glutamine synthetase, inactivated in vivo, was activated 8-fold by thioredoxin and dithioerythrol. By the same treatment, the activity of glutamate synthase was stimulated nearly 4-fold. Thus, two key enzymes of N metabolism were shown to be regulated via thioredoxin. The enzymes of the nitrate reducing system, i.e. nitrate reductase and nitrite reductase, were not affected by thiols. From these results, a model of NO3− metabolism is put forward which considers the regulating effect of light. |
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