Distribution of incorporated, synthetic cytokinins in ribosomal RNA preparations from tobacco callus |
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| Authors: | Murai N Armstrong D J Taller B J Skoog F |
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| Affiliation: | Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706. |
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| Abstract: | The distribution of incorporated synthetic cytokinins (N6-8-14C]benzyladenine (8-14C]bzl6Ade) and N68-14C]furfuryladenine (8-14C]fr6Ade) in ribosomal RNA prepared from tobacco callus (Nicotiana tabacum L. var. Wis. No. 38) grown in the presence of one of these for 25 or 26 days has been studied. The rRNA of tissue supplied with 8-14C]bzl6Ade or 8-14C]fr6Ade was fractionated by methylated albumin-Kieselguhr column chromatography and preparative gel electrophoresis, respectively. In each case about 80% of the incorporated cytokinin was recovered as the ribonucleoside 8-14C]bzl6A or 8-14C]fr6A in the rRNA peak after the fractionations. 8-14C]fr6A was found associated with both the 18S and 25S rRNA components in quantities roughly proportional to their 260 nm absorbance. This pattern of apparently nonspecific association was not affected by prior denaturation of the RNA with formamide. |
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