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Accelerated Plaque Formation by Fowlpox Virus in the Presence of Chymotrypsin |
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| Authors: | Bonnie B Asch and George E Gifford |
| Abstract: | Chymotrypsin enhanced fowlpox virus plaque formation in chick embryo cell cultures. A simplified plaque assay for fowlpox virus is described. Plaques were produced in 3 days when chymotrypsin was included in a serum-free fluid overlay. Plaques were also produced in 5 to 6 days under an agar overlay when a medium containing fetal calf serum was employed. Kinetics of plaque formation were also studied, and it was shown that fowlpox virus plaque diameters grow at a linear rate. |
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