Effect of Ca2+ ions on plasmid transformation of Streptococcus lactis protoplasts. |
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| Authors: | A von Wright A M Taimisto and S Sivel |
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| Affiliation: | A von Wright, A M Taimisto, and S Sivelä |
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| Abstract: | The effects of Mg2+ and Ca2+ ions on the efficiency of the plasmid transformation of lysozyme-treated Streptococcus lactis protoplasts were compared. A 33-megadalton plasmid, pLP712, coding for lactose fermentation and a 6.5-megadalton plasmid, pGB301, coding for erythromycin and chloramphenicol resistance were used as model plasmids, and S. lactis MG1614 was the recipient. Replacing Mg2+ with Ca2+ in the transformation buffer was found to increase transformant frequency more than 10-fold with both plasmids. |
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