Regulated expression of the Escherichia coli dam gene |
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| Authors: | Calmann Melissa A Marinus M G |
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| Affiliation: | Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. |
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| Abstract: | ![]()
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease. |
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