Multi‐isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen |
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| Authors: | Eugene Boon Beng Ong Joshua Ignatius Amy Amilda Anthony Ismail Aziah Asma Ismail Theam Soon Lim |
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| Affiliation: | 1. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Malaysia;2. School of Mathematical Sciences, Universiti Sains Malaysia, Minden, Malaysia |
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| Abstract: | The detection and measurement of different antibody isotypes in the serum provide valuable indicators of the different stages of typhoid infection. Here, the ability of S. Typhi recombinant hemolysin E (HlyE) to detect multi‐isotype antibody responses in sera of patients with typhoid and paratyphoid A was investigated using an indirect antibody immunoassay. Nanogram amounts of HlyE were found to be sufficient for detection of IgG and IgA isotypes and, in a study of individuals' sera (n = 100), the immunoassay was able to distinguish between typhoid and non‐typhoid sera. The overall sensitivity, specificity and efficiency of the ELISA were 70% (39/56), 100% (44/44) and 83% respectively. |
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| Keywords: | antibody isotypes ELISA recombinant hemolysin E antigen Salmonella Typhi |
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