Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine. |
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| Authors: | D Fernandez-Forner Y Palom S Ikuta E Pedroso and R Eritja |
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| Affiliation: | Department of Organic Chemistry, University of Barcelona, Spain. |
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| Abstract: | A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethylthymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphitetriester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed. |
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