Liquid chromatographic separation and thermodynamic investigation of lorcaserin hydrochloride enantiomers on immobilized amylose–based chiral stationary phase |
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| Authors: | Dattatraya V Wani Vipul P Rane Santosh N Mokale |
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| Affiliation: | 1. Department of Pharmaceutical Chemistry, Maulana Azad Education Trust's Y.B. Chavan College of Pharmacy, Aurangabad, Maharashtra, India;2. Wockhardt Research Centre Aurangabad, Aurangabad, Maharashtra, India |
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| Abstract: | A novel liquid chromatographic method was developed for enantiomeric separation of lorcaserin hydrochloride on Chiralpak IA column containing chiral stationary phase immobilized with amylose tris (3.5‐dimethylphenylcarbamate) as chiral selector. Baseline separation with resolution greater than 4 was achieved using mobile phase containing mixture of n‐hexane/ethanol/methanol/diethylamine (95:2.5:2.5:0.1, v/v/v/v) at a flow rate of 1.2 mL/min. The limit of detection and limit of quantification of the S‐enantiomer were found to be 0.45 and 1.5 μg/mL, respectively; the developed method was validated as per ICH guideline. The influence of column oven temperatures studied in the range of 20°C to 50°C on separation was studied; from this, retention, separation, and resolution were investigated. The thermodynamic parameters ΔH°, ΔS°, and ΔG° were evaluated from van't Hoff plots,(Ink′ versus 1/T) and used to explain the strength of interaction between enantiomers and immobilized amylose–based chiral stationary phase |
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| Keywords: | enantiomer and chiral separation immobilized amylose lorcaserin hydrochloride van't Hoff plots |
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