| 缓慢干旱下春小麦叶片质膜脂肪酸组成、H~+-ATPase肥及5′-AMPase活力的变化 |
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| 引用本文： | 宫海军,陈坤明,陈国仓,朱学艺,王锁民,张承烈.缓慢干旱下春小麦叶片质膜脂肪酸组成、H~+-ATPase肥及5′-AMPase活力的变化[J].植物生态学报,2003(4). |
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| 作者姓名： | 宫海军 陈坤明 陈国仓 朱学艺 王锁民 张承烈 |
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| 作者单位： | 兰州大学生命科学学院，兰州大学生命科学学院，兰州大学生命科学学院，兰州大学生命科学学院，兰州大学生命科学学院，兰州大学生命科学学院 兰州 730000，兰州 730000 天水师范学院生物系，甘肃天水 741001，兰州 730000，兰州 730000，兰州 730000，兰州 730000 |
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| 基金项目： | 国家重点基础研究规划项目(G1999011705)，国家科技攻关计划项目(2001BA901A18) |
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| 摘 要： | 研究了田间缓慢干旱胁迫下,抗旱性不同的两个小麦(Triticum aestivum)品种的生长状况、质膜极性脂脂肪酸组成以及质膜关键酶活力的变化。在小麦生长发育的早期,干旱胁迫使其叶片质膜极性脂脂肪酸不饱和度下降、质膜微囊消耗O_2的速率升高、膜蛋白含量降低、H~+-ATPase(EC 3.6.1.35)活力下降、5′-AMPase(EC 3.1.3.5)活力大幅度升高;在小麦发育的后期,随着干旱的持续,小麦叶片质膜的极性脂脂肪酸不饱和度不变或升高、质膜微囊消耗O_2的速率降低、膜蛋白含量与H~+-ATPase活力升高、5′-AMPase活力下降。以上结果表明,小麦在发育的早期阶段对干旱较敏感,其细胞质膜流动性降低、细胞中能荷贮备降低;而在后期,则又表现出对干旱的适应。这些结果将有助于阐明自然干旱条件下植物的抗旱机制。
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| 关 键 词： | 小麦 缓慢干旱 质膜 脂肪酸 关键酶 |
EFFECTS OF GRADUAL DROUGHT ON THE FATTY ACID COMPOSITION OF POLAR LIPIDS, H~+ -ATPase AND 5'-AMPase ACTIVITIES IN THE PLASMA MEMBRANES OF TWO SPRING WHEAT LEAVES |
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| GONGHai-Jun CHEN Kun-Ming CHEN Guo-Cang ZHU Xue-Yi,WANG Suo-Min and ZHANG Cheng-Lie.EFFECTS OF GRADUAL DROUGHT ON THE FATTY ACID COMPOSITION OF POLAR LIPIDS, H~+ -ATPase AND 5'-AMPase ACTIVITIES IN THE PLASMA MEMBRANES OF TWO SPRING WHEAT LEAVES[J].Acta Phytoecologica Sinica,2003(4). |
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| Authors: | GONGHai-Jun CHEN Kun-Ming CHEN Guo-Cang ZHU Xue-Yi WANG Suo-Min and ZHANG Cheng-Lie |
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| Abstract: | Previous studies on responses of plants to drought stress were mostly performed with young plant seedlings subjected to experimental stress. Experimental stresses are usually abruptly imposed on plants. However, in the drought field, plants are subjected to gradual stress because water availability in the soil does not change quickly. Therefore, responses of plants might be different under the two stress conditions. The plasma membrane is very sensitive to drought, which causes alteration of its structure and functions. To our knowledge , few studies concerning the effects of gradual field drought on properties of the plasma membrane in the course of whole developmental stages have been published. In the present work, we took two spring wheat ( Triticum aestivum) as materials and investigated changes in plant growth, fatty acid composition of polar lipids and activities of key enzymes in the plasma membranes of leaves under gradual field drought. Drought stress decreased water content of wheat leaves and inhibited plant growth. At early developmental stages, drought stress decreased fatty acid unsaturation of polar lipids in the plasma membranes, membrane protein content and activities of H + -ATPase (EC 3.6.1.35), while it increased 5'-AMPase (EC 3.1.3.5) activity and O2 resumption by plasma membranes. At late developmental stages, with drought progressing, membrane protein content and H+ -ATPase activities were increased by continuing drought, and fatty acid unsaturation of polar lipids in the plasma membranes was increased or not significantly changed, whereas 5'-AMPase activities and O2 resumption by plasma membranes were decreased. These results suggest that wheat plants are sensitive to drought at early developmental stages, and the fluidity of plasma membranes as well as energy reservoirs decrease. At late stages, adaptive phenomena of plants to drought were observed. Our results might be able to help elucidate plant drought resistance under natural drought conditions. |
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| Keywords: | Wheat Gradual drought Plasma membrane Fatty acid Key enzymes |
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