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Studies on the Bohr Effect of Sheep Hemoglobin |
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| Authors: | Derek G Smyth Frederick C Battaglia and Giacomo Meschia |
| Abstract: | A simple method is described for the measurement of pH changes in hemoglobin solutions on oxygenation and reduction. Data are presented establishing the absence of a Bohr effect in p-chloromercuribenzoate PCMB] treated hemoglobin. The influence of a number of sulfhydryl inhibitors on the Bohr effect of a hemoglobin solution is reported and an interpretation based on steric factors in the protein is proposed. |
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