Dual recombinant Lactococcus lactis for enhanced delivery of DNA vaccine reporter plasmid pPERDBY |
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| Authors: | Bhrugu Yagnik Drashya Sharma Harish Padh Priti Desai |
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| Affiliation: | 1. Department of Cell and Molecular Biology, B. V. Patel Pharmaceutical Education and Research Development Centre, Ahmedabad, Gujarat;2. B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidhyanagar, Gujarat;3. Sardar Patel University, Vallabh Vidhyanagar, Gujarat, India |
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| Abstract: | Food grade Lactococcus lactis has been widely used as an antigen and DNA delivery vehicle. We have previously reported the use of non‐invasive L. lactis to deliver the newly constructed immunostimulatory DNA vaccine reporter plasmid, pPERDBY. In the present report, construction of dual recombinant L. lactis expressing internalin A of Listeria monocytogenes and harboring pPERDBY (LL InlA + pPERDBY) to enhance the efficiency of delivery of DNA by L. lactis is outlined. After confirmation and validation of LL InlA + pPERDBY, its DNA delivery potential was compared with previously developed non‐invasive r‐ L. lactis::pPERDBY. The use of invasive L. lactis resulted in around threefold increases in the number of enhanced green fluorescent protein‐expressing Caco‐2 cells. These findings reinforce the prospective application of invasive strain of L. lactis for delivery of DNA/RNA and antigens. |
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| Keywords: | DNA delivery enhanced green fluorescent protein invasive Lactococcus lactis |
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