The self‐sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5‐hydroxylation of diclofenac |
| |
Authors: | Jan M Klenk Bernd A Nebel Joanne L Porter Justyna K Kulig Shaneela A Hussain Sven M Richter Michele Tavanti Nicholas J Turner Martin A Hayes Bernhard Hauer Sabine L Flitsch |
| |
Affiliation: | 1. Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany;2. School of Chemistry, Manchester Institute of Biotechnology, The University of Manchester, Manchester, UK;3. Cardiovascular and Metabolic Diseases DMPK, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, M?lndal, Sweden;4. Present address: Crop Science Division, Bayer AG, Monheim am Rhein, Germany |
| |
Abstract: | P450 monooxygenases are able to catalyze the highly regio‐ and stereoselective oxidations of many organic molecules. However, the scale‐up of such bio‐oxidations remains challenging due to the often‐low activity, level of expression and stability of P450 biocatalysts. Despite these challenges they are increasingly desirable as recombinant biocatalysts, particularly for the production of drug metabolites. Diclofenac is a widely used anti‐inflammatory drug that is persistent in the environment along with the 4'‐ and 5‐hydroxy metabolites. Here we have used the self‐sufficient P450 RhF (CYP116B2) from Rhodococcus sp. in a whole cell system to reproducibly catalyze the highly regioselective oxidation of diclofenac to 5‐hydroxydiclofenac. The product is a human metabolite and as such is an important standard for environmental and toxicological analysis. Furthermore, access to significant quantities of 5‐hydroxydiclofenac has allowed us to demonstrate further oxidative degradation to the toxic quinoneimine product. Our studies demonstrate the potential for gram‐scale production of human drug metabolites through recombinant whole cell biocatalysis. |
| |
Keywords: | Biocatalysis Cytochrome P450 Diclofenac Drug metabolism Fermentation Regioselectivity |
|
|