Endogenous gibberellins of a radiation induced single gene dwarf mutant of bean |
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Authors: | Proano V A Greene G L |
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Affiliation: | Nuclear Energy Program, Training and Research Center, Interamerican Institute of Agricultural Sciences of the Organization of American States, Turrialba, Costa Rica. |
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Abstract: | The distribution of endogenous gibberellins in Dwarf-1, a single gene dwarf mutant of Mexico 80-R red beans, was studied. Parallel extraction and fractionation of seeds of this mutant and those of a normal homozygous line followed by thin layer chromatography and bioassays using Rumex obtusifolius, wheat seed endosperm and dwarf bean plants revealed that a stem elongation control factor was contained in the non-acidic fraction from normal, but not from Dwarf-1, seeds. It was concluded that the single gene mutation causes a block either in gibberellin precursor formation or in production of a non-acidic fraction gibberellin-like substance. |
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