Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse. |
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| Authors: | D G Wilkinson G Peters C Dickson and A P McMahon |
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| Affiliation: | Laboratory of Developmental Biochemistry, National Institute for Medical Research, Mill Hill, London, UK. |
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| Abstract: | The proto-oncogene int-2 has been implicated in the formation of mouse mammary-tumour-virus-induced mammary tumours. Analysis of the predicted coding sequence indicates that int-2 is a member of the fibroblast growth factor family. Previous studies using Northern blot analysis suggested that normal expression of int-2 may be confined to extra-embryonic endoderm lineages of embryonic stages of mouse development. We have used in situ hybridization and Northern blot analysis to examine directly int-2 expression in embryo stem cells and in the developing embryo from early gastrulation to midsomite stages. Complex patterns of accumulation of int-2 RNA were observed in embryonic and extra-embryonic tissues. The data suggest multiple roles for int-2 in development which may include migration of early mesoderm cells and induction of the otocyst. |
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