Guanine tracts enhance sequence directed DNA bends. |
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| Authors: | D L Milton M L Casper N M Wills and R F Gesteland |
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| Affiliation: | Howard Hughes Medical Institute Laboratory, Department of Human Genetics, University of Utah, Salt Lake City 84132. |
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| Abstract: | Synthetic DNA fragments were constructed to determine the effect of G tracts, in conjunction with periodically spaced A tracts, on DNA bends. Relative length measurements showed that the G tracts spaced at the half helical turn enhanced the DNA bend. When the G tract was interrupted with a thymine or shortened to one or two guanines, the relative lengths decreased. If the G tract was replaced with either an A tract or a T tract, the bend was cancelled. Replacement with a C tract decreased the relative length to that of a thymine interruption suggesting that bend enhancement due to G tracts requires A tracts on the same strand. |
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