The oxidation of malate and exogenous reduced nicotinamide adenine dinucleotide by isolated plant mitochondria |
| |
| Authors: | Day D A Wiskich J T |
| |
| Affiliation: | Department of Botany, University of Adelaide, Adelaide, 5001, Australia. |
| |
| Abstract: | Exogenous NADH oxidation by cauliflower (Brassica oleracea L.) bud mitochondria was sensitive to antimycin A and gave ADP/O ratios of 1.4 to 1.9. In intact mitochondria, NADH-cytochrome c reductase activity was only slightly inhibited by antimycin A. The antimycin-insensitive activity was associated with the outer membrane. Malate oxidation was sensitive to both rotenone and antimycin A and gave ADP/O values of 2.4 to 2.9. However in the presence of added NAD+, malate oxidation displayed similar properties to exogenous NADH oxidation. In both the presence and absence of added NAD+, malate oxidation was dependent on inorganic phosphate and inhibited by 2-n-butyl malonate. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
