1. Physics Department, International Laser Center, M.V. Lomonosov Moscow State University, Moscow, Russia;2. Kurchatov Institute National Research Center, Moscow, Russia;3. Department of Physics and Astronomy, Texas A&M University, College Station TX, USA;4. Russian Quantum Center, Skolkovo, Moscow Region, Russia;5. Kazan Quantum Center, A. N. Tupolev Kazan National Research Technical University, Kazan, Russia;6. P.K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia
Abstract:
Optical coupling between a single, individually addressable neuron and a properly designed optical fiber is demonstrated. Two‐photon imaging is shown to enable a quantitative in situ analysis of such fiber–single‐neuron coupling in the live brain of transgenic mice. Fiber‐optic interrogation of single pyramidal neurons in mouse brain cortex is performed with the positioning of the fiber probe relative to the neuron accurately mapped by means of two‐photon imaging. These results pave the way for fiber‐optic interfaces to single neurons for a stimulation and interrogation of individually addressable brain cells in chronic in vivo studies on freely behaving transgenic animal models, as well as the integration of fiber‐optic single‐neuron stimulation into the optical imaging framework.
