Large-Molecular-Weight Precursors of Sindbis Virus Proteins |
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| Authors: | Milton J Schlesinger and Sondra Schlesinger |
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| Affiliation: | 1Department of Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110 |
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| Abstract: | Infection of chicken embryo fibroblasts with a temperature-sensitive mutant of Sindbis virus at the nonpermissive temperature leads to the accumulation of a large-molecular-weight protein. We have shown that this protein contains (14)C-arginine tryptic peptides present in the three virion proteins. We have also found that a slightly smaller protein which is detected in Sindbis-infected BHK cells contains the (14)C-arginine tryptic peptides of the two envelope proteins but not those of the capsid protein. Pulse-chase experiments indicate that the Sindbis virus protein in BHK cells is cleaved to the envelope proteins. |
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