Response of carbon dioxide fixation to water stress: parallel measurements on isolated chloroplasts and intact spinach leaves |
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| Authors: | Plaut Z Bravdo B |
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| Affiliation: | Agricultural Research Organization, The Volcani Center, Bet Dagan. |
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| Abstract: | Application of water stress to isolated spinach (Spinacia oleracea) chloroplasts by redutcion of the osmotic potentials of CO2 fixation media below −6 to −8 bars resulted in decreased rates of fixation regardless of solute composition. A decrease in CO2 fixation rate of isolated chloroplasts was also found when leaves were dehydrated in air prior to chloroplast isolation. An inverse response of CO2 fixation to osmotic potential of the fixation medium was found with chloroplasts isolated from dehydrated leaves—namely, fixation rate was inhibited at −8 bars, compared with −16 or −24 bars. |
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