Bioorganic Research of Galactites tomentosa Moench. Honey Extracts: Enantiomeric Purity of Chiral Marker 3‐Phenyllactic Acid |
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| Authors: | Igor Jerković Marin Roje Carlo I G Tuberoso Zvonimir Marijanović Ana Kasum Marina Obradović |
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| Affiliation: | 1. Department of Organic Chemistry, Faculty of Chemistry & Technology, University of Split, Split, Croatia;2. Division of Organic Chemistry and Biochemistry, Rudjer Boskovic Institute, Zagreb, Croatia;3. Department of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy;4. Department of Food Technology, Polytechnic " Marko Marulic" in Knin, Knin, Croatia |
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| Abstract: | Thistle (Galactites tomentosa Moench.) honey organic extracts were obtained by headspace solid‐phase microextraction (HS‐SPME) and ultrasonic solvent extraction (USE) and analyzed by gas chromatography (GC‐FID and GC‐MS) for the first time. Most abundant headspace compounds were terpenes, particularly linalool derivatives (hotrienol was predominant with a range of 38.6–57.5%). 3‐Phenyllactic acid dominated in the solvent extracts (77.4–86.4%) followed by minor percentages of other shikimate pathway derivatives. After determination of an adequate enantioseparation protocol on Chirallica PST‐4 column, the honey solvent extracts were analyzed by high‐performance liquid chromatography (HPLC). The chiral analysis revealed high enantiomeric excess (>95%) of (–)‐3‐phenyllactic acid in all samples. Therefore, previous findings of chemical markers of thistle honey were extended, providing new potential for advanced chemical fingerprinting (optical pure chemical marker). Chirality 26:405–410, 2014. © 2014 Wiley Periodicals, Inc. |
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| Keywords: | volatile organic compounds GC‐FID GC‐MS chiral HPLC with polarimetric detector (– )‐3‐phenyllactic acid |
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