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Influence of Protein Synthesis on NO(3) Reduction, NH(4) Accumulation, and Amide Synthesis in Suspension Cultures of Paul's Scarlet Rose
Authors:Bradford J A  Fletcher J S
Affiliation:Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019.
Abstract:Changes in the concentrations of NH4+ and amides during the growth of suspension cultures of rose (Rosa cv. Paul's Scarlet) cells were examined. When cells were grown in medium possessing only NO3 as a nitrogen source, the concentrations of NH4+ and amides increased to 4.0 × 10−1 and 5.9 micromoles per gram fresh weight, respectively. The amounts of both constituents declined during the later stages of growth. When a trace amount of NH4+ was added to the NO3 base starting medium, the concentration of NH4+ in the cells was increased to 7.0 × 10−1 micromoles per gram fresh weight.
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