Preparation and characterization of antigen and antibody absorbents covalently coupled to an inorganic carrier |
| |
| Authors: | Howard H. Weetall |
| |
| Affiliation: | Research and Development Laboratories, Corning Glass Works, Corning, N.Y. 14830, U.S.A. |
| |
| Abstract: | Immunoadsorbents were prepared by the use of an inorganic carrier. The proteins were covalently coupled to the carrier through a silane coupling agent. An immunoadsorbent prepared with human gamma-globulin was found to complex with specific antibodies. These antibodies could be recovered in high yield and purity from the adsorbent. Similarly, insolubilized antibodies to l-asparaginase were used to isolate and purify the enzyme from crude extracts. These insolubilized antibodies are extremely stable with time and may be reused many times. |
| |
| Keywords: | |
|
|
