Direct repeats flanking the Bacteroides transposon Tn4351 are insertion sequence elements. |
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| Authors: | V Hwa N B Shoemaker and A A Salyers |
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| Affiliation: | Department of Microbiology, University of Illinois, Urbana 61801. |
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| Abstract: | The clindamycin-erythromycin resistance (Ccr Emr) region of the Bacteroides transposon Tn4351 is flanked by direct repeats. This study showed that the direct repeats are insertion sequence (IS) elements. Although both IS elements can mediate transfer of the chloramphenicol (Cmr) marker on pBR328 by cointegrate formation with the conjugal IncW plasmid R388, IS4351R-mediated transfer of Cmr occurred at a consistently lower frequency than did the transfer mediated by IS4351L. Analysis of plasmids from the resultant transconjugants revealed IS-mediated activities such as deletions, tandem duplication of IS4351L, and excision of IS4351R. |
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