Polyphosphoinositides are present in plasma membranes isolated from fusogenic carrot cells |
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| Authors: | Wheeler J J Boss W F |
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| Affiliation: | Department of Botany, North Carolina State University, Raleigh, North Carolina 27695. |
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| Abstract: | Fusogenic carrot cells grown in suspension culture were labeled 12 hours with myo-2-3H]inositol. Plasma membranes were isolated from the prelabeled fusogenic carrot cells by both aqueous polymer two-phase partitioning and Renografin density gradients. With both methods, the plasma membrane-enriched fractions, as identified by marker enzymes, were enriched in 3H]inositol-labeled phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP2). An additional 3H]inositol-labeled lipid, lysophosphatidylinositol monophosphate, which migrated between PIP and PIP2 on thin layer plates, was found primarily in the plasma membrane-rich fraction of the fusogenic cells. This was in contrast to lysophosphatidylinositol which is found primarily in the lower phase, microsomal/mitochrondrial-rich fraction. |
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