Predictions of matrix‐assisted refolding of α‐lactalbumin: Process efficiency versus batch dilution method |
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| Authors: | Sylwia Ry? Wojciech Pi?tkowski Dorota Antos |
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| Affiliation: | Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów, Poland |
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| Abstract: | Protein refolding is an important technique to produce active recombinant proteins from inclusion bodies. Because of the complexity of the refolding process, a trial‐and‐error method is usually used for its design, which is ineffective and time consuming. Therefore, an efficient method for the process prediction is indispensable to optimize the operating conditions. In this article, we suggest a design procedure for matrix‐assisted protein refolding. Three different chromatographic techniques were considered exploiting hydrophobic interaction chromatography, ion‐exchange chromatography, and SEC media. The procedure consisted of quantification of refolding kinetics, analysis of the retention behavior of all protein forms involved in refolding, construction of a dynamic model, and the process simulation. Denatured bovine α‐lactalbumin was used as model protein. The refolding rate was measured for different protein concentration using the batch dilution method. A kinetic scheme for the protein refolding was suggested and incorporated into a dynamic model of chromatographic column and used for predicting the refolding performance. The productivity, yield, and buffer consumption were used as performance indicators for the refolding techniques considered. The matrix‐assisted protein refolding process outperformed batch dilution method with respect to all indicators provided that efficient method for the process design was used. |
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| Keywords: | Batch dilution Matrix‐assisted refolding Process dynamics Protein chromatography Protein refolding |
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