Anopheles gambiae miRNAs as actors of defence reaction against Plasmodium invasion |
| |
| Authors: | Winter Flore Edaye Sonia Hüttenhofer Alexander Brunel Christine |
| |
| Affiliation: | Flore Winter, Sonia Edaye, Alexander Hüttenhofer, and Christine Brunel |
| |
| Abstract: | The path Plasmodium takes across the Anopheles midgut constitutes the major bottleneck during the malaria transmission cycle. In the present study, using a combination of shot-gun cloning and bioinformatic analysis, we have identified 18 miRNAs from Anopheles gambiae including three miRNAs unique to mosquito. Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system. Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium. We also show that knocking down Dicer1 and Ago1 mRNAs led to an increased sensitivity to Plasmodium infection. Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
