Following Suberization in Potato Wound Periderm by Histochemical and Solid-State 13C Nuclear Magnetic Resonance Methods |
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| Authors: | Stark R E Sohn W Pacchiano Jr R A Al-Bashir M Garbow J R |
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| Affiliation: | Department of Chemistry, College of Staten Island and the Graduate School of the City University of New York, 2800 Victory Boulevard, Staten Island, New York 10314 (R.E.S., W.S., R.A.P., M.A.-B). |
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| Abstract: | The time course of suberization in wound periderm from potato (Solanum tuberosum L.) has been monitored by histochemical and high-resolution solid-state nuclear magnetic resonance (NMR) methods. Light microscopy conducted after selective staining of the lipid and double-bonded constituents shows that suberin is deposited at the outermost intact cell-wall surface during the first 7 d of wound healing; suberization forms a barrier to tissue infiltration at later times. Cross polarization-magic angle spinning 13C NMR spectra demonstrate the deposition of a polyester containing all major suberin functional groups after just 4 d of wound healing. Initially the suberin includes a large proportion of aromatic groups and fairly short aliphatic chains, but the spectral data demonstrate the growing dominance of long-chain species during the period 7 to 14 d after wounding. The results of preliminary 13C-labeling experiments with sodium 2-13C]acetate and DL-1-13C]phenylalanine provide an excellent prospectus for future NMR-based studies of suberin biosynthesis. |
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