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Escherichia coli tryptophan operon directs the in vivo synthesis of a leader peptide. |
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| Authors: | L Dekel-Gorodetsky R Schoulaker-Schwarz H Engelberg-Kulka |
| Abstract: | Here we report the identification of the Escherichia coli trp leader peptide synthesized in vivo. We identified the peptide in UV-irradiated maxicells by selective labeling with radioactive amino acids which are included in the predicted sequence of this peptide. Our results support the hypothesis that translation of the peptide-coding region of the leader RNA has a role in the mechanism of attenuation of biosynthetic operons in general and in the E. coli trp operon in particular. |
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