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Detection of Listeria monocytogenes by using the polymerase chain reaction.
Authors:M T Bessesen  Q A Luo  H A Rotbart  M J Blaser  and R T Ellison  rd
Affiliation:M T Bessesen, Q A Luo, H A Rotbart, M J Blaser, and R T Ellison, 3rd
Abstract:A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
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