Detection of Listeria monocytogenes by using the polymerase chain reaction. |
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Authors: | M T Bessesen Q A Luo H A Rotbart M J Blaser and R T Ellison rd |
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Affiliation: | M T Bessesen, Q A Luo, H A Rotbart, M J Blaser, and R T Ellison, 3rd |
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Abstract: | A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains. |
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