| Abstract: | Based on pharmacological evidence, we previously proposed that intracellular Ca2+ mediates the perception of O2 deprivation in maize seedlings. Herein, using fluorescence imaging and photometry of Ca2+ in maize suspension-cultured cells, the proposal was further investigated. Two complementary approaches were taken: (1) real time analysis of anoxia-induced changes in cytosolic Ca2+ concentration (Ca]i) and (2) experimental manipulation of Ca]i and then assay of the resultant anoxia-specific responses. O2 depletion caused an immediate increase in Ca2+]i, and this was reversible within a few seconds of reoxygenation. The Ca]i elevation proceeded independent of extracellular Ca2+. The kinetics of the Ca2+ response showed that it occurred much earlier than any detectable changes in gene expression. Ruthenium red blocked the anoxic Ca]i elevation and also the induction of adh1 (encoding alcohol dehydrogenase) and sh1 (encoding sucrose synthase) mRNA. Ca2+, when added along with ruthenium red, prevented the effects of the antagonist on the anoxic responses. Verapamil and bepridil failed to block the Ca]i rise induced by anoxia and were equally ineffective on anoxic gene expression. Caffeine induced an elevation of Ca]i as well as ADH activity under normoxia. The data provide direct evidence for Ca]i elevation in maize cells as a result of anoxia-induced mobilization of Ca2+ from intracellular stores. Furthermore, any manipulation that modified the Ca]i rise brought about a parallel change in the expression of two anoxia-inducible genes. Thus, these results corroborate our proposal that Ca]i is a physiological transducer of anoxia signals in plants. |
