Characterization of a Novel Angular Dioxygenase from Fluorene-Degrading Sphingomonas sp. Strain LB126 |
| |
| Authors: | Luc Schuler Sinad M Ní Chadhain Yves Jouanneau Christine Meyer Gerben J Zylstra Pascal Hols and Spiros N Agathos |
| |
| Affiliation: | Luc Schuler, Sinéad M. Ní Chadhain, Yves Jouanneau, Christine Meyer, Gerben J. Zylstra, Pascal Hols, and Spiros N. Agathos |
| |
| Abstract: | In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The α and β subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran degrader Terrabacter sp. strain DBF63, were identified. When overexpressed in Escherichia coli, FlnA1-FlnA2 was responsible for the angular oxidation of fluorene, 9-hydroxyfluorene, 9-fluorenone, dibenzofuran, and dibenzo-p-dioxin. Moreover, FlnA1-FlnA2 was able to oxidize polycyclic aromatic hydrocarbons and heteroaromatics, some of which were not oxidized by the dioxygenase from Terrabacter sp. strain DBF63. The quantification of resulting oxidation products showed that fluorene and phenanthrene were the preferred substrates of FlnA1-FlnA2. |
| |
| Keywords: | |
|
|
