Elevation of the cytosolic free [Ca2+] is indispensable for the transduction of the Nod factor signal in alfalfa. |
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| Authors: | H H Felle E Kondorosi A Kondorosi M Schultze |
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| Affiliation: | Botanisches Institut I, Justus-Liebig-Universit?t, Senckenbergstrasse 17, D-35390 Giessen, Germany. hubert.felle@bio.uni-giessen.de |
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| Abstract: | In root hairs of alfalfa (Medicago sativa), the requirement of Ca(2+) for Nod factor signaling has been investigated by means of ion-selective microelectrodes. Measured 50 to 100 microm behind the growing tip, 0.1 microM NodRm-IV(C16:2,S) increased the cytosolic free Ca2+] by about 0.2 pCa, while the same concentration of chitotetraose, the nonactive glucosamine backbone, had no effect. We demonstrate that NodRm-IV(C16:2,S) still depolarized the plasma membrane at external Ca(2+) concentrations below cytosolic values if the free EGTA concentration remained low (
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