Interactions of donor sources and media influence the histo‐morphological quality of full‐thickness skin models |
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| Authors: | Julia Lange Frederik Weil Christoph Riegler Florian Groeber Silke Rebhan Szymon Kurdyn Miriam Alb Hermann Kneitz Götz Gelbrich Heike Walles Stephan Mielke |
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| Affiliation: | 1. Department of Internal Medicine II, Center for Allogeneic Stem Cell Transplantation, Würzburg University Medical Center, Würzburg, Germany;2. Institute of Clinical Epidemiology and Biometry, University of Würzburg, Würzburg, Germany;3. Department of Tissue Engineering and Regenerative Medicine, Würzburg University Medical Center, Würzburg, Germany;4. Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskeletal Disease, Würzburg Branch of the Fraunhofer‐Institute Interfacial Engineering and Biotechnology, IGB, Würzburg, Germany;5. Department of Dermatology, Section for Histopathology, Würzburg University Medical Center, Würzburg, Germany |
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| Abstract: | Human artificial skin models are increasingly employed as non‐animal test platforms for research and medical purposes. However, the overall histopathological quality of such models may vary significantly. Therefore, the effects of manufacturing protocols and donor sources on the quality of skin models built‐up from fibroblasts and keratinocytes derived from juvenile foreskins is studied. Histo‐morphological parameters such as epidermal thickness, number of epidermal cell layers, dermal thickness, dermo‐epidermal adhesion and absence of cellular nuclei in the corneal layer are obtained and scored accordingly. In total, 144 full‐thickness skin models derived from 16 different donors, built‐up in triplicates using three different culture conditions were successfully generated. In univariate analysis both media and donor age affected the quality of skin models significantly. Both parameters remained statistically significant in multivariate analyses. Performing general linear model analyses we could show that individual medium‐donor‐interactions influence the quality. These observations suggest that the optimal choice of media may differ from donor to donor and coincides with findings where significant inter‐individual variations of growth rates in keratinocytes and fibroblasts have been described. Thus, the consideration of individual medium‐donor‐interactions may improve the overall quality of human organ models thereby forming a reproducible test platform for sophisticated clinical research. |
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| Keywords: | Biomaterials Cell culture Tissue engineering Translational medic |
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