| 不同强度运动训练对心肌凋亡途径微小RNA及其靶蛋白的影响 |
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| 引用本文： | 赵永才,付近梅,高炳宏.不同强度运动训练对心肌凋亡途径微小RNA及其靶蛋白的影响[J].中国应用生理学杂志,2018,34(1):93-96. |
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| 作者姓名： | 赵永才 付近梅 高炳宏 |
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| 作者单位： | 1. 上海体育学院, 上海 200438;
2. 江西省体育科学研究所, 南昌 330006 |
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| 基金项目： | 上海市科学技术委员会重点攻关项目（15dz1208001）；上海市人类运动能力开发与保障重点实验室（17DZ2273100） |
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| 摘 要： | 目的：考察不同负荷运动训练对小鼠心肌凋亡相关miR-1，miR-21和靶蛋白的影响，探讨运动干预心肌凋亡的可能机制。方法：选取21只C57BL/6小鼠，随机分为3组（n=7）：安静组（SE组）、训练1组（ET1组）、训练2组（ET2）。SE组不进行训练，ET1组完成8周递增负荷游泳训练，5天/周，1次/天，第1周30 min/count，每周增加10 min，第7、8周时间维持在90 min；ET2组在ET1组方案基础上增加负荷，前5周与ET1相同，后3周每天训练2次。TUNEL检测考察心肌凋亡水平，Western blot和RT-PCR分别测定蛋白和miRs的变化。结果：ET1组游泳训练对小鼠心肌凋亡影响不明显，miR-1表达无显著变化，但其靶蛋白Bcl-2表达显著增高（P<0.01），miR-21及其靶蛋白PDCD4表达均无显著变化。ET2组游泳训练显著降低心肌凋亡水平及miR-1表达（P<0.01）、提高Bcl-2表达（P<0.05）；同时显著提高miR-21表达（P<0.05），但对PDCD4表达无明显影响。结论：ET1组训练对心肌凋亡干预不明显，ET2组运动训练可降低心肌凋亡水平，miR-1及靶蛋白Bcl-2变化可能是机制之一，PDCD4对运动训练不敏感，miR-21可能与其它靶蛋白参与运动干预心肌凋亡的分子机制。
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| 关 键 词： | 运动 心肌 微小RNA 凋亡 小鼠 |
| 收稿时间： | 2016-10-09 |
Effects of different intensity exercise training on apoptosis-related microRNAs and the targeted proteins in cardiomyocytes |
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| ZHAO Yong-cai,FU Jin-mei,GAO Bing-hong.Effects of different intensity exercise training on apoptosis-related microRNAs and the targeted proteins in cardiomyocytes[J].Chinese Journal of Applied Physiology,2018,34(1):93-96. |
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| Authors: | ZHAO Yong-cai FU Jin-mei GAO Bing-hong |
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| Affiliation: | 1. Shanghai University of Sport, Shanghai 200438;
2. Institute for Sports Science of Jiangxi Province, Nanchang 330006, China |
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| Abstract: | Objective: To detect the levels of miR-1, miR-21 and their targeted proteins in hearts of mice after different exercise training, and discuss potential molecular mechanism.Methods: Male C57BL/6 mice were randomly divided to 3 groups:sedentary (SE), exercise training 1(ET1) and exercise training 2 (ET2). SE did not do any exercise; ET1 undertook swimming training for 8 weeks, once a day, 5 days/week. Swimming 30 min in the 1st week, and the duration was increased 10 min per week to 90 min and maintained in the 7th and 8th week. ET2 performed the same work as ET1 and switched to twice a day by the end of the 5th week. TUNEL assay was applied to test myocardial apoptosis. Western blot and RT-PCR were used to detect proteins and miRs levels respectively.Results: Compared with SE, in ET1, myocardial apoptosis and miR-1 level did not change, but its targeted protein Bcl-2 increased significantly(P<0.01). miR-21 and its targeted protein PDCD4 did not change significantly. In ET2, myocardial apoptosis and miR-1 level were decreased significantly(P<0.05). Bcl-2 was increased significantly(P<0.01). miR-21 also increased significantly (P<0.05), but PDCD4 did not decrease significantly.Conclusion: Exercise training in ET2 other than ET1 could down-regulate myocardial apoptosis. Alterations of miR-1 and Bcl-2 may be responsible for this cardioprotection. PDCD4 is not sensitive to exercise training, it is likely that miR-21 and other targeted proteins participate in exercise-regulative apoptosis. |
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| Keywords: | exercise cardiomyocyte microRNAs apoptosis mice |
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