The von Hippel-Lindau tumor suppressor protein controls ciliogenesis by orienting microtubule growth |
| |
| Authors: | Schermer Bernhard Ghenoiu Cristina Bartram Malte Müller Roman Ulrich Kotsis Fruzsina Höhne Martin Kühn Wolfgang Rapka Manuela Nitschke Roland Zentgraf Hanswalter Fliegauf Manfred Omran Heymut Walz Gerd Benzing Thomas |
| |
| Affiliation: | Renal Division and 2Children's Hospital, University Hospital Freiburg, 79106 Freiburg, Germany. |
| |
| Abstract: | Cilia are specialized organelles that play an important role in several biological processes, including mechanosensation, photoperception, and osmosignaling. Mutations in proteins localized to cilia have been implicated in a growing number of human diseases. In this study, we demonstrate that the von Hippel-Lindau (VHL) protein (pVHL) is a ciliary protein that controls ciliogenesis in kidney cells. Knockdown of pVHL impeded the formation of cilia in mouse inner medullary collecting duct 3 kidney cells, whereas the expression of pVHL in VHL-negative renal cancer cells rescued the ciliogenesis defect. Using green fluorescent protein-tagged end-binding protein 1 to label microtubule plus ends, we found that pVHL does not affect the microtubule growth rate but is needed to orient the growth of microtubules toward the cell periphery, a prerequisite for the formation of cilia. Furthermore, pVHL interacts with the Par3-Par6-atypical PKC complex, suggesting a mechanism for linking polarity pathways to microtubule capture and ciliogenesis. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
